Directing phage selections towards specific epitopes
نویسندگان
چکیده
منابع مشابه
Directing the outcome of deoxyribozyme selections to favor native 3'-5' RNA ligation.
Previous experiments have identified numerous RNA ligase deoxyribozymes, each of which can synthesize either 2',5'-branched RNA, linear 2'-5'-linked RNA, or linear 3'-5'-linked RNA. These products may be formed by reaction of a 2'-hydroxyl or 3'-hydroxyl of one RNA substrate with the 5'-triphosphate of a second RNA substrate. Here the inherent propensities for nucleophilic reactivity of specifi...
متن کاملPhage display identifies two Caprine Arthritis Encephalitis Virus env epitopes
Using phage display and IgG of a goat infected with Caprine Arthritis Encephalitis Virus (CAEV) we obtained families of 7 mer constrained peptides with consensus motifs LxSDPF/Y and SWN/KHWSY and mapped the epitopes mimicked by them at the Env 6-LISDPY-11 and 67-WNTYHW-72 sites of the mature gp135 amino acid sequence. The first epitope fell into the N-terminal immunogenic aa1-EDYTLISDPYGFS- aa1...
متن کاملAutomated Detection of Conformational Epitopes Using Phage Display Peptide Sequences
BACKGROUND Precise determination of conformational epitopes of neutralizing antibodies represents a key step in the rational design of novel vaccines. A powerful experimental method to gain insights on the physical chemical nature of conformational epitopes is the selection of linear peptides that bind with high affinities to a monoclonal antibody of interest by phage display technology. Howeve...
متن کاملPhage Displayed Short Peptides against Cells of Candida albicans Demonstrate Presence of Species, Morphology and Region Specific Carbohydrate Epitopes
Candida albicans is a commensal opportunistic pathogen, which can cause superficial infections as well as systemic infections in immuocompromised hosts. Among nosocomial fungal infections, infections by C. albicans are associated with highest mortality rates even though incidence of infections by other related species is on the rise world over. Since C. albicans and other Candida species differ...
متن کاملSelections
It was not until the sixteenth century that dead bodies were submitted to medico-legal investigation. The horror which human dissections inspired in the ancients, and the religious awe with which they beheld the mortal wreck of their fellows, not only retarded the progress of anatomy and physiology, but excluded the light of medicine from the researches of the forum. The Jewish law, indeed, con...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: "Protein Engineering, Design and Selection"
سال: 1996
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/9.11.1043